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Accession number: 20094712458877（检索号）

Title: Preparation of monoclonal antibody against okadaic acid and development of an sensitive ELISA for detection diarrhetic shellfish poisoning in shellfish

Authors: Liu, Renyan1 ; Xu, Daoyan1 ; Liang, Yubo1

Author affiliation: 1 National Marine Environmental Monitoring Center, Dalian 116023, China

Corresponding author: Liu, R. (ryliu@nmemc.gov.cn)

Source title: Proceedings - 2009 International Conference on Environmental Science and Information Application Technology, ESIAT 2009

Abbreviated source title: Proc. - Int. Conf. Environ. Sci. Inf. Appl. Technol., ESIAT

Volume: 2

Monograph title: Proceedings - 2009 International Conference on Environmental Science and Information Application Technology, ESIAT 2009

Issue date: 2009

Publication year: 2009

Pages: 550-555

Article number: 5199952

Language: English

ISBN-13: 9780769536828

Document type: Conference article (CA)

Conference name: 2009 International Conference on Environmental Science and Information Application Technology, ESIAT 2009

Conference date: July 4, 2009 - July 5, 2009

Conference location: Wuhan, China

Conference code: 77943

Publisher: IEEE Computer Society, 445 Hoes Lane - P.O.Box 1331, Piscataway, NJ 08855-1331, United States

Abstract: A rapid method for detection diarrhetic shellfish poisoning (DSP),a marine biotoxin,were developed.Monoclonal antibody against OA(okadaic acid) was prepared by using the technique of cell fusion.This antibody was applied in development the idc-ELISA(indirect competitive enzyme-linked immunosorbent assay) for detection of OA.Using OA-KLH as the immunogen to immunize BALB/c mice,splenocytes from the immunized mice were fused with murine myeloma cells.Three positive hybridoma cells strains that could secrete steadily anti-OA monoclonal antibody were obtained after three times of cloning.The idc-ELISA method with detection limit of 0.781ng/ml was developed using 1#monoclonal antibody showing highest specificity against OA. Okadaic acid added was detected with average recoveries of 92.5% for scallop.
Number of references: 18